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Macvector plasmid mapping
Macvector plasmid mapping









macvector plasmid mapping

Manna D, Breier AM, Higgins NP (2004) Microarray analysis of transposition targets in Escherichia coli: the impact of transcription.

macvector plasmid mapping

Liu G, Geurts AM, Yae K, Srinivasan AR, Fahrenkrug SC, Largaespada DA et al (2005) Target-site preferences of sleeping beauty transposons. Lawrence JG, Ochman H (1997) Amelioration of bacterial genomes: rates of change and exchange. Koonin EV, Wolf YI (2008) Genomics of bacteria and archaea: the emerging dynamic view of the prokaryotic world. Kholodii GY, Gorlenko Z, Lomovskaya OL, Mindlin SZ, Yurieva OV, Nikiforov VG (1993) Molecular characterization of an aberrant mercury resistance transposable element from an environmental Acinetobacter strain. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Jeon CO, Park W, Ghiorse WC, Madsen EL (2004) Polaromonas naphthalenivorans sp.

macvector plasmid mapping

Studies on the microorganisms of cereal grain. Ito H, Iizuka H (1971) Taxonomic studies on a radio-resistant Pseudomonas. Heuer H, Kopmann C, Binh TT, Top EM, Smalla K (2009) Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low % G + C content. Gstalder ME, Faelen M, Mine N, Top EM, Mergeay M, Couturier M (2003) Replication functions of new broad host range plasmids isolated from polluted soils. Harwood Academic Publishers, Amsterdam, pp 1–47įrost LS, Leplae R, Summers AO, Toussaint A (2005) Mobile genetic elements: the agents of open source evolution. In: Thomas CM et al (eds) The horizontal gene pool: bacterial plasmids and gene spread. doi: 10.1016/S0168-6496(98)00085-3Įspinosa M, Cohen SN, Couturier M, del Solar G, Diaz-Orejas R, Giraldo R (2000) Plasmid replication and copy number control. J Bacteriol 172:6568–6572ĭrønen AK, Torsvik V, Goksøyr J, Top EM (1998) Effect of mercury addition on plasmid incidence and gene mobilising capacity in bulk soil. doi: 10.1101/gr.2289704ĭe Lorenzo V, Herrero M, Jakubzik U, Timmis KN (1990) Mini-Tn 5 transposon derivatives for insertion mutagenesis, promotor probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. doi: 10.1038/35082545ĭarling AC, Mau B, Blattner FR, Perna NT (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements. doi: 10.1080/09687860500063316Ĭoates JD, Chakraborty R, Lack JG, O’Connor SM, Cole KA, Bender KS, Achenbach LA (2001) Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas. doi: 10.1128/AEM.-8655.2005Ĭhristie PJ, Cascales E (2005) Structural and dynamic properties of bacterial type IV secretion systems. doi: 10.1073/pnas.Ĭhakraborty R, O’Connor SM, Chan E, Coates JD (2005) Anaerobic degradation of benzene, toluene, ethylbenzene, and xylene compounds by Dechloromonas strain RCB.

macvector plasmid mapping

doi: 10.1093/nar/Ĭampbell A, Mrazek J, Karlin S (1999) Genome signature comparisons among prokaryote, plasmid, and mitochondrial DNA. doi: 10.1093/nar/gnh080īurland V, Shao Y, Perna NT, Plunkett G, Sofia HJ, Blattner FR (1998) The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. doi: 10.1093/nar/Īson B, Reznikoff WS (2004) A high-throughput assay for Tn 5 Tnp-induced DNA cleavage. The presence of very similar BHR plasmids with different accessory genes in geographically distinct locations suggests an important role in horizontal gene exchange and bacterial adaptation for this recently defined plasmid group, which we propose to name “PromA”.Īltschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. This demonstrates the necessity for careful screening of plasmids and host chromosomes to avoid mis-interpretation of plasmid genome content. The transposon unique to pMOL98 appears to have inserted from the chromosome of the recipient strain used in the plasmid capture procedure. Comparison of the variable accessory regions of these plasmids shows that the majority of natural transposons, as well as the mini-transposon used to mark the plasmids, are inserted in the parA locus. They share a plasmid backbone comprising replication, partitioning and conjugative transfer functions. Here, complete sequence analysis and comparative genomics reveal that the 55.5 kb nucleotide sequence of pMOL98 shows extensive sequence similarity and synteny with the BHR plasmid family that now includes pIPO2, pSB102, pTER331, and pMRAD02. The self-transmissible, broad-host-range (BHR) plasmid pMOL98 was previously isolated from polluted soil using a triparental plasmid capture approach and shown to possess a replicon similar to that of the BHR plasmids pSB102 and pIPO2.











Macvector plasmid mapping